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KMID : 0545120200300081214
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Journal of Microbiology and Biotechnology
2020 Volume.30 No. 8 p.1214 ~ p.1221
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Apoptosis in Leukemic Cells Induced by Anti-proliferative Coumarin Isolated from the Stem Bark of Fraxinus rhynchophylla
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Lee Beom-Zoo
Lee Ik-Soo
Pham Chau-Ha
Jeong Soon-Kyu
Lee Sul-Hae
Hong Kwang-Won
Yoo Hee-Min
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Abstract
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Esculetin 6-O-β-D-arabinofuranosyl-(1 →6)-β-D-glucopyranoside (EAG) is a coumarin glycoside isolated from the stem bark of Fraxinus rhynchophylla. This study scrutinized the anti-proliferative activity of EAG on blood cancer-derived Jurkat leukemic cells. Cell viability assays in leukemic cancer cells determined that EAG possesses potent anti-proliferative effects. Moreover, treatment with EAG increased the proportion of apoptotic cells, resulted in cell cycle arrest being induced at the subG0/ G1 phase, and reduced the proportion of cells present in the S phase. In addition, mitochondrial membrane potential was reduced by EAG in Jurkat cells. Additionally, EAG triggered apoptosis that was mediated by the downregulation of BCL-XL, p-IκBα, and p-p65 expressions in addition to the upregulation of cleaved Caspase 3 and BAX expressions. These findings revealed that the toxic effect of EAG was mediated by intracellular signal transduction pathways that involved a mechanism in which reactive oxygen species (ROS) were upregulated. Thus, this study concludes that EAG could potentially serve as a therapeutic agent for leukemia.
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KEYWORD
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Fraxinus rhynchophylla, coumarin, leukemia, apoptosis, ROS, cytotoxicity
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